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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # LON20/H1

Format

BSA Free

Concentration

1 mg/ml

Product Summary for LONP1 Antibody (LON20/H1) - BSA Free

Immunogen

KLH-peptide conjugate - N-CEKDDKDAIEEKFRERLKE-C

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LONP1 Antibody (LON20/H1) - BSA Free

Western Blot: LONP1 Antibody (LON20/H1)BSA Free [NBP2-50478]

Western Blot: LONP1 Antibody (LON20/H1)BSA Free [NBP2-50478]

Western Blot: LONP1 Antibody (LON20/H1) [NBP2-50478] - U87 cells were transfected with siRNA against the Lon protease (siLonp1.1 and siLonp1.2) or a control sequence (siControl) or transfection reagent alone (mock) and then western blotted using anti-LONP1 [LON20/H1] and a loading control antibody (B-actin). Molecular weight markers are labelled in kilodaltons

Applications for LONP1 Antibody (LON20/H1) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Positive control(s): U87 cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LONP1

LONP1 encodes a mitochondrial matrix protein in the Lon family of ATP-dependent proteases. A similar E. coli protein regulates gene expression by targeting specific regulatory proteins for degradation. This protein binds a specific sequence in the light and heavy chain promoters of the mitochondrial genome which are involved in regulation of DNA replication and transcription. [provided by RefSeq]

Alternate Names

EC 3.4.21, EC 3.4.21.-, EC 3.4.21.53, hLON, hLON ATP-dependent protease, LON, lon peptidase 1, mitochondrial, lon protease homolog, mitochondrial, Lon protease-like protein, LONHs, LONP, Mitochondrial ATP-dependent protease Lon, mitochondrial lon peptidase 1, mitochondrial lon protease-like protein, PIM1, protease, serine, 15, PRSS15MGC1498, Serine protease 15

Gene Symbol

LONP1

Additional LONP1 Products

Product Documents for LONP1 Antibody (LON20/H1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LONP1 Antibody (LON20/H1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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