LONP1 Antibody

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734]

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human fallopian tube shows granular cytoplasmic positivity in glandular cells.

Western Blot: LONP1 Antibody [NBP1-81734]

Western Blot: LONP1 Antibody [NBP1-81734] - PINK1 accumulates upon knockdown of Lon. Western blot analysis of Lon protease (Lon), Mitochondrial transcription factor A (TFAM), Heat shock protein 60 (Hsp60) and Actin in whole head homogenate from control and Lon deficient animals. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1004279), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: LONP1 Antibody [NBP1-81734]

Immunocytochemistry/Immunofluorescence: LONP1 Antibody [NBP1-81734] - Staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734]

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human tonsil shows granular cytoplasmic positivity.

Western Blot: LONP1 Antibody [NBP1-81734]

Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734]

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human pancreas shows granular cytoplasmic positivity.

Simple Western: LONP1 Antibody [NBP1-81734]

Simple Western: LONP1 Antibody [NBP1-81734] - Electropherogram image(s) of corresponding Simple Western lane view. LONP1 antibody was used at 1:20 dilution on h. Kidney and NIH-3T3 lysate(s).

Western Blot: LONP1 Antibody [NBP1-81734] -

Western Blot: LONP1 Antibody [NBP1-81734] - MTO1 defective cells exhibit proteostasis stress and an altered bioenergetic state. Representative immunoblot showing the expression of LONP1 in extracts of WT and MTO1 HF. Porin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29348686/) licensed under a CC-BY license.

Western Blot: LONP1 Antibody [NBP1-81734] -

Western Blot: LONP1 Antibody [NBP1-81734] - Altered mitochondrial features in cybrid cells carrying MELAS, MERRF, m.5514 A > G (mt-tRNATrp), m.1643A > G (mt-tRNAVal), & m.14487 T > C (ND6) mutations. (A) Representative western blot of LONP1, AFG3L2 & CLPP peptidases in mutant & wild type (WT) cybrid cells. The membrane was also probed with porin as a loading control. Full-length western blots & lower-exposure blots of porin are included in supplementary information. (B) Densitometric analysis of LONP1, AFG3L2 & CLPP normalized to porin & represented as fold change relative to WT (top). Quantitative data are from at least three independent experiments. Results from this analysis are also shown as a heatmap (bottom). The color & the corresponding value in log2 scale are depicted on the left. (C) Representative Blue Native-PAGE of OXPHOS complexes in mutant & WT cybrid cells. Full-length blots & lower-exposure blots for those with high contrast are included in supplementary information. (D) Densitometric analysis of OXPHOS complexes normalized to complex-II (loading control) & represented as fold change relative to WT. (E) Cellular ATP determination in mutant & WT cybrid cells. (F & G) Determination of Ca2+ (F) & ROS (G) by flow cytometry in mutant & WT cybrid cells with Fluo-3 & MitoSOX Red, respectively. All data are the mean ± SEM of at least three different experiments. Differences from WT values were found to be statistically significant at *p < 0.05, **p < 0.01 & ***p < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28740091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.