LOX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24877

Novus Biologicals, part of Bio-Techne
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NBP2-24877
NBP2-24877SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Sheep

Cited:

Human, Mouse, Rat, Porcine, Ovine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Lysyl Oxidase/LOX Antibodies »

Product Specifications

Immunogen

A portion of amino acids 305-338 of human LOX protein were used as the immunogen.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 26224524). Sheep reactivity reported in scientific literature (PMID: 26563105).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LOX Antibody

Western Blot: LOX Antibody [NBP2-24877]

Western Blot: LOX Antibody [NBP2-24877]

Western Blot: LOX Antibody [NBP2-24877] - Analysis of mouse ear lysate when LOX antibody (NBP2-24877) was used at 1:1000. The band shown is the expected inactive 50 kD proenzyme.
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NBP2-24877]

Immunocytochemistry/ Immunofluorescence: LOX Antibody [NBP2-24877]

Immunocytochemistry/Immunofluorescence: LOX Antibody [NBP2-24877] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LOX (NBP2-24877) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: LOX Antibody [NBP2-24877]

Immunohistochemistry-Paraffin: LOX Antibody [NBP2-24877]

Immunohistochemistry-Paraffin: LOX Antibody [NBP2-24877] - Analysis of LOX in formalin-fixed, paraffin-embedded mouse skin using an isotype control (top) and NBP2-24877 (bottom) at 1:100.
View 1 more images

Applications for LOX Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

reported in scientific literature (PMID 29404848)

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Knockout Validated

reported in scientific literature (PMID 31173582)

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

0.1 ml crude whole Rabbit sera with 50% Glycerol

Preservative

0.025% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lysyl Oxidase/LOX

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Long Name

Lysyl Oxidase

Alternate Names

AAT10

Gene Symbol

LOX

Additional Lysyl Oxidase/LOX Products

Product Documents for LOX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LOX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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