LRCH3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81083
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HASPLPPSAAPTTDSTDSITGQNSRQREEELELIDQLRKHIEYRLKVSLPCDLGAALTDGVVLCHLANHVRPRSVPSIHVPSP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LRCH3 Antibody
Western Blot: LRCH3 Antibody [NBP1-81083]
Western Blot: LRCH3 Antibody [NBP1-81083] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Immunocytochemistry/ Immunofluorescence: LRCH3 Antibody [NBP1-81083]
Immunocytochemistry/Immunofluorescence: LRCH3 Antibody [NBP1-81083] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]
Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.Applications for LRCH3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRCH3
Alternate Names
FLJ20994, FLJ43245, FLJ44976,2210409B11Rik, leucine-rich repeat and calponin homology domain-containing protein 3, leucine-rich repeats and calponin homology (CH) domain containing 3, MGC4126
Entrez Gene IDs
84859 (Human)
Gene Symbol
LRCH3
Additional LRCH3 Products
Product Documents for LRCH3 Antibody
Product Specific Notices for LRCH3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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