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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human LRCH4 (NP_002310) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

73.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LRCH4 Antibody (OTI1F8) - Azide and BSA Free

Western Blot: LRCH4 Antibody (OTI1F8)Azide and BSA Free [NBP2-72520]

Western Blot: LRCH4 Antibody (OTI1F8)Azide and BSA Free [NBP2-72520]

Western Blot: LRCH4 Antibody (OTI1F8) - Azide and BSA Free [NBP2-72520] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRCH4.
Immunohistochemistry: LRCH4 Antibody (OTI1F8) - Azide and BSA Free [NBP2-72520]

Immunohistochemistry: LRCH4 Antibody (OTI1F8) - Azide and BSA Free [NBP2-72520]

Immunohistochemistry: LRCH4 Antibody (OTI1F8) - Azide and BSA Free [NBP2-72520] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LRCH4 Antibody (OTI1F8) - Azide and BSA Free [NBP2-72520]

Immunohistochemistry: LRCH4 Antibody (OTI1F8) - Azide and BSA Free [NBP2-72520]

Immunohistochemistry: LRCH4 Antibody (OTI1F8) - Azide and BSA Free [NBP2-72520] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for LRCH4 Antibody (OTI1F8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LRCH4

The LRCH4 gene encodes a protein that contains leucine-rich repeats (LRR) at its amino terminus and that is known to be involved in ligand binding. The carboxyl terminus may act as a membrane anchor. Identified structural elements suggest that the encoded prot

Alternate Names

FLJ40101, FLJ46315, leucine rich repeat neuronal 4, Leucine-rich neuronal protein, leucine-rich repeat and calponin homology domain-containing protein 4, Leucine-rich repeat neuronal protein 4, leucine-rich repeats and calponin homology (CH) domain containing 4, LRN, LRRN1, LRRN4, PP14183

Gene Symbol

LRCH4

Additional LRCH4 Products

Product Documents for LRCH4 Antibody (OTI1F8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRCH4 Antibody (OTI1F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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