LRH-1/NR5A2 Antibody (JE30-50)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32660
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE30-50
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human LRH-1/NR5A2 aa 442-541 / 541. (Uniprot: O00482)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LRH-1/NR5A2 Antibody (JE30-50)
Western Blot: LRH-1/NR5A2 Antibody (JE30-50) [NBP3-32660]
Western Blot: LRH-1/NR5A2 Antibody (JE30-50) [NBP3-32660] - Western blot analysis of LRH-1/NR5A2 on different lysates with Rabbit anti-LRH-1/NR5A2 antibody (NBP3-32660) at 1/1,000 dilution.Lane 1: MCF7 cell lysate (15 ug/Lane)
Lane 2: 293T cell lysate (15 ug/Lane)
Lane 3: HepG2 cell lysate (15 ug/Lane)
Lane 4: A549 cell lysate (15 ug/Lane)
Lane 5: PANC-1 cell lysate (15 ug/Lane)
Lane 6: SW1990 cell lysate (15 ug/Lane)
Lane 7: Mouse liver tissue lysate (30 ug/Lane)
Lane 8: Rat liver tissue lysate (30 ug/Lane)
Lane 9: Mouse brain tissue lysate (30 ug/Lane)
Lane 10: Rat brain tissue lysate (30 ug/Lane)
Lane 11: Mouse pancreas tissue lysate (30 ug/Lane)
Predicted band size: 61 kDa
Observed band size: 65 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for LRH-1/NR5A2 Antibody (JE30-50)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRH-1/NR5A2
Long Name
Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2
Alternate Names
B1F2, FTZ-F1 beta, LRH1, NR5A2
Gene Symbol
NR5A2
Additional LRH-1/NR5A2 Products
Product Documents for LRH-1/NR5A2 Antibody (JE30-50)
Product Specific Notices for LRH-1/NR5A2 Antibody (JE30-50)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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