Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all LRH-1/NR5A2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free

Western Blot: LRH-1/NR5A2 Antibody (OTI3H8)Azide and BSA Free [NBP2-72521]

Western Blot: LRH-1/NR5A2 Antibody (OTI3H8)Azide and BSA Free [NBP2-72521]

Western Blot: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.
Immunohistochemistry: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521]

Immunohistochemistry: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521]

Immunohistochemistry: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LRH-1/NR5A2

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.

Long Name

Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2

Alternate Names

B1F2, FTZ-F1 beta, LRH1, NR5A2

Gene Symbol

NR5A2

Additional LRH-1/NR5A2 Products

Product Documents for LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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