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LRP2 Antibody (10B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26163

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-26163-100ul
NBP3-26163-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10B5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for LRP2 Antibody (10B5)

Immunogen

A synthesized peptide derived from Human LRP2 [UniProt P98164]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRP2 Antibody (10B5)

LRP2 Antibody (10B5)

Flow Cytometry: LRP2 Antibody (10B5) [NBP3-26163] -

Flow Cytometry: LRP2 Antibody (10B5) [NBP3-26163] - Overlay Peak curve showing MCF7 cells stained with LRP2 Antibody (10B5) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for LRP2 Antibody (10B5)

Application
Recommended Usage

Flow Cytometry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Megalin/LRP2

Megalin/gp330, an approx. 600 kDa transmembrane glycoprotein is a large cell surface receptor that mediates the binding and internalization of a number of structurally and functionally distinct ligands from the lipoprotein and protease inhibitor families.

Long Name

LDL Receptor-related Protein 2

Alternate Names

DBS, gp330, LRP-2, LRP2

Gene Symbol

LRP2

Additional Megalin/LRP2 Products

Product Documents for LRP2 Antibody (10B5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRP2 Antibody (10B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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