LRP2 Antibody (CD7D5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80836

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CD7D5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Megalin/LRP2 Antibodies »

Product Summary for LRP2 Antibody (CD7D5) - Azide and BSA Free

Immunogen

Purified human Megalin

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

600 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LRP2 Antibody (CD7D5) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836]

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836]

Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-LRP2 (CD7D5) at 20 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836]

Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836]

Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836] - Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836]

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836]

Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NBP2-80836] - Immunofluorescence staining of Megalin in the brush border of the proximal tubule. Image from the standard format of this antibody.
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Applications for LRP2 Antibody (CD7D5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunohistochemistry

2-5 ug/ml

Immunohistochemistry-Frozen

1:50-1:300

Immunohistochemistry-Paraffin

1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Megalin/LRP2

Megalin/gp330, an approx. 600 kDa transmembrane glycoprotein is a large cell surface receptor that mediates the binding and internalization of a number of structurally and functionally distinct ligands from the lipoprotein and protease inhibitor families.

Long Name

LDL Receptor-related Protein 2

Alternate Names

DBS, gp330, LRP-2, LRP2

Gene Symbol

LRP2

Additional Megalin/LRP2 Products

Product Documents for LRP2 Antibody (CD7D5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LRP2 Antibody (CD7D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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