LRPAP Antibody (4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89796

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LRPAP Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human LRPAP (Tyr 35-Leu 357; Accession#: NP_002328.1).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for LRPAP Antibody (4)

Western Blot: LRPAP Antibody (4) [NBP2-89796]

Western Blot: LRPAP Antibody (4) [NBP2-89796]

Western Blot: LRPAP Antibody (4) [NBP2-89796] - Lane A: U87-MG Whole Cell Lysate Lane B: PC-3 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDa Observed band size:41 kDa
Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (4) [NBP2-89796]

Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (4) [NBP2-89796]

Immunocytochemistry/Immunofluorescence: LRPAP Antibody (4) [NBP2-89796] - Staining of Human LRPAP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LRPAP1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation: LRPAP Antibody (4) [NBP2-89796]

Immunoprecipitation: LRPAP Antibody (4) [NBP2-89796]

Immunoprecipitation: LRPAP Antibody (4) [NBP2-89796] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-LRPAP1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LRPAP1 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size: 41 kDa

Applications for LRPAP Antibody (4)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRPAP

Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330

Long Name

Low Density Lipoprotein-Related Protein-associated Protein

Alternate Names

A2MRAP, A2RAP, HBP44, LRPAP1, RAP

Gene Symbol

LRPAP1

Additional LRPAP Products

Product Documents for LRPAP Antibody (4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LRPAP Antibody (4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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