LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12047
Recombinant Monoclonal Antibody
![Western Blot: LRPAP Antibody (7F1)ChimericAzide and BSA Free [NBP3-12047]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-7F1-Chimeric-Western-Blot-NBP3-12047-img0001.jpg "Western Blot: LRPAP Antibody (7F1)ChimericAzide and BSA Free [NBP3-12047]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 7F1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free
Immunogen
This antibody was raised by immunising mice with human placental LRPAP.
Specificity
This antibody is specific for human LRPAP. It does not cross-react with rabbit or murine LRPAP.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free
Western Blot: LRPAP Antibody (7F1)ChimericAzide and BSA Free [NBP3-12047]
Western Blot: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Staining in HeLa(A) (0.1ug/ml), A549 (B) (0.01ug/ml), HepG2 (C) (0.01ug/ml), HEK293 (D) (0.03ug/ml), MCF7 (E) (0.03ug/ml), Caco-2 (F) (0.001ug/ml) and U251 (G) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAP (NBP3-12047) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-7F1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12047-img0002.jpg "Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047]")
Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047]
Immunocytochemistry/Immunofluorescence: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 7F1 (NBP3-12047) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: LRPAP
Long Name
Low Density Lipoprotein-Related Protein-associated Protein
Alternate Names
A2MRAP, A2RAP, HBP44, LRPAP1, RAP
Gene Symbol
LRPAP1
Additional LRPAP Products
Product Documents for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free
Product Specific Notices for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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