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LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12047

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 7F1

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

This antibody was raised by immunising mice with human placental LRPAP.

Specificity

This antibody is specific for human LRPAP. It does not cross-react with rabbit or murine LRPAP.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Western Blot: LRPAP Antibody (7F1)ChimericAzide and BSA Free [NBP3-12047]

Western Blot: LRPAP Antibody (7F1)ChimericAzide and BSA Free [NBP3-12047]

Western Blot: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Staining in HeLa(A) (0.1ug/ml), A549 (B) (0.01ug/ml), HepG2 (C) (0.01ug/ml), HEK293 (D) (0.03ug/ml), MCF7 (E) (0.03ug/ml), Caco-2 (F) (0.001ug/ml) and U251 (G) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAP (NBP3-12047) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047]

Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047]

Immunocytochemistry/Immunofluorescence: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 7F1 (NBP3-12047) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: LRPAP

Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330

Long Name

Low Density Lipoprotein-Related Protein-associated Protein

Alternate Names

A2MRAP, A2RAP, HBP44, LRPAP1, RAP

Gene Symbol

LRPAP1

Additional LRPAP Products

Product Documents for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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