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LRRC3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88289

Novus Biologicals, part of Bio-Techne
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NBP1-88289
NBP1-88289-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GAVAVFCSLRGLQEVPEDIPANTVLLKLDANKISHLPDGAFQHLHRLRELDLSHNAIEAIGSATFAGLAGGLRLLDLSYNRIQRIPKDALGKLSAKIRLSHNPLHCECALQEALWELKLDPDSVDEIACHTSVQEEFVGKP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRRC3 Antibody

Western Blot: LRRC3 Antibody [NBP1-88289]

Western Blot: LRRC3 Antibody [NBP1-88289]

Western Blot: LRRC3 Antibody [NBP1-88289] - Analysis in control (vector only transfected HEK293T lysate) and LRRC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: LRRC3 Antibody [NBP1-88289]

Immunohistochemistry-Paraffin: LRRC3 Antibody [NBP1-88289]

Immunohistochemistry-Paraffin: LRRC3 Antibody [NBP1-88289] - Staining of human breast shows cytoplasmic positivity in glandular cells.

Applications for LRRC3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRRC3

LRRC3 (Leu-rich repeat/LRR-containing protein 3) is a 25 kDa (predicted) member of the Leu-rich repeat protein superfamily. Little is known about the protein except for the fact that it shows widespread expression. Human LRRC3 is a protein that is 225 amino acids (aa) in length (SwissProt #:Q9BY71). It contains three LRRs between aa 63-86, aa 87-110, and aa 112-135. There is one potential splice variant that shows a deletion of aa 129-248. Over aa 33-204, human LRRC3 is 83% and 81% aa identical to mouse and canine LRRC3, respectively.

Long Name

Leucine-rich Repeat Containing 3

Alternate Names

C21orf102, chromosome 21 open reading frame 102, leucine rich repeat containing 3, leucine-rich repeat-containing protein 3, LRRC3A

Gene Symbol

LRRC3

Additional LRRC3 Products

Product Documents for LRRC3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRRC3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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