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LRRC50 Antibody (OTI2C4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01936

Novus Biologicals, part of Bio-Techne
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NBP2-01936
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C4

Concentration

2.74 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human LRRC50(NP_848547) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

79.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LRRC50 Antibody (OTI2C4)

Western Blot: LRRC50 Antibody (2C4) [NBP2-01936] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow Cytometry: LRRC50 Antibody (OTI2C4) [NBP2-01936]

Flow Cytometry: LRRC50 Antibody (OTI2C4) [NBP2-01936]

Flow Cytometry: LRRC50 Antibody (2C4) [NBP2-01936] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LRRC50 antibody, and then analyzed by flow cytometry.
Western Blot: LRRC50 Antibody (OTI2C4) [NBP2-01936]

Western Blot: LRRC50 Antibody (OTI2C4) [NBP2-01936]

Western Blot: LRRC50 Antibody (2C4) [NBP2-01936] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.

Applications for LRRC50 Antibody (OTI2C4)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

2.74 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LRRC50

The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. COMPLETENESS: complete on the 3' end.

Alternate Names

CILD13, DKFZp434A119, FLJ25330, leucine rich repeat containing 50, ODA7leucine-rich repeat-containing protein 50, outer row dynein assembly 7 homolog

Entrez Gene IDs

123872 (Human)

Gene Symbol

DNAAF1

Additional LRRC50 Products

Product Documents for LRRC50 Antibody (OTI2C4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRRC50 Antibody (OTI2C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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