LRRK2 Antibody (S138-6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42175
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S138-6
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein, amino acids 1-500 (N-terminus) of human LRRK2. 83% identical in mouse and rat. No significant identity with LRRK1.
Reactivity Notes
Immunogen displays sequence identity for non-tested species: Mouse and Rat.
Localization
Cytoplasm , Membrane , Mitochondrion , Golgi Apparatus , Cell Projection , Axon , Dendrite , Endoplasmic Reticulum , Cytoplasmic Vesicle , Endosome , Lysosome
Specificity
Detects >200kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for LRRK2 Antibody (S138-6)
Western Blot: LRRK2 Antibody (S138-6) [NBP2-42175]
Western Blot: LRRK2 Antibody (S138-6) [NBP2-42175] - Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (NBP2-42175). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (NBP2-42175) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa.Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175]
Immunocytochemistry/Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (NBP2-42175). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (NBP2-42175) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRRK2/Dardarin Antibody (D) Composite.Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175]
Immunocytochemistry/Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X.Applications for LRRK2 Antibody (S138-6)
Application
Recommended Usage
Western Blot
1:1000
Application Notes
1 ug/ml of LRRK2/Dardarin Antibody was sufficient for detection of LRRK2/Dardarin in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRRK2
LRRK2 antibodies are useful tools for neurodegeneration research and certian kinase signaling pathway studies.
Long Name
Leucine-rich Repeat Ser/Thr Protein Kinase 2
Alternate Names
AURA17, Dardarin, PARK8, ROCO2
Gene Symbol
LRRK2
UniProt
Additional LRRK2 Products
Product Documents for LRRK2 Antibody (S138-6)
Product Specific Notices for LRRK2 Antibody (S138-6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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