LRRK2 Antibody

Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771]

Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771] - DAB Immunostaining in frozen sections of mouse brainstem.

Knockdown Validated: LRRK2 Antibody [NB110-58771]

Knockdown Validated: LRRK2 Antibody [NB110-58771] - LRRK2 RNA levels 5 days after shRNA lentiviral transduction. The empty vector was used as the mock control. Lines represent the mean +/- SEM of three to four independent silencing experiments. A representative immunoblot analysis of LRRK2 protein levels 5 days after transduction is also shown. Beta-III-tubulin was the loading control. Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x) licensed under a CC-BY license.

Western Blot: LRRK2 Antibody [NB110-58771] -

Western Blot: LRRK2 Antibody [NB110-58771] - Characterization of iPSC-derived DA neurons with LRRK2 mutations. a Diagram showing the DA differentiation protocol used for neural induction of human iPSC lines. b Temporal gene expression analyzed by qRT-PCR at three time points: induction (3 weeks), expansion (4–5 weeks), & maturation (>6 weeks). Each point represents the mean ± SEM of at least two independent differentiation experiments. c Representative images of mature neuronal cultures showing expression of neuronal ( betaIII-tubulin, Tau, & alpha-synuclein) & dopaminergic (TH, NURR1) markers. Nuclei were counterstained with Hoechst. Scale bars: 50 μm. d Quantification of immunostainings. Data are represented as mean ± SEM of counts from at least two different lines for each genotype. e Representative western blot analyses of TH, Tau, & GFAP with betaIII-tubulin as loading control in iPSC-derived mature neurons. f Representative immunoblots & quantification of LRRK2 expression in mature neuronal cultures. alpha-tubulin was the loading control & data were normalized to control WT neurons. Bars represent the mean ± SEM of at least two different lines per genotype. DIV days in vitro, GEL gelatin, POL poly-ornithine, FBN fibronectin, LMN laminin, N2 N2 supplement, bFGF basic fibroblast growth factor, SAG smoothened agonist, LDN LDN-193189, CHIR CHIR99021, SB SB431542, BDNF brain-derived neurotrophic factor, AA ascorbic acid, B27 B27 supplement, dbcAMP dibutyryl cyclic adenosine monophosphate, TGF betaIII transforming growth factor betaIII, GDNF glial derived neurotrophic factor. See Additional file 2 for uncropped blots Image collected & cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.