LRRK2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78299

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all LRRK2 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal portion of the human LRRK2 protein (between residues 500-650) [UniProt# Q5S007]

Localization

Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRRK2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody - BSA Free [NBP1-78299]

Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody - BSA Free [NBP1-78299]

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NBP1-78299] - LRRK2 antibody was tested at 1:100 in U2OS cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: LRRK2 Antibody - BSA Free [NBP1-78299]

Immunohistochemistry: LRRK2 Antibody - BSA Free [NBP1-78299]

Immunohistochemistry: LRRK2 Antibody [NBP1-78299] - Analysis of LRRK2 in mouse brain using DAB with hematoxylin counterstain.

Applications for LRRK2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:300

Immunohistochemistry-Paraffin

1:300
Application Notes
This LRRK2 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, mitochondrial staining is observed in U2OS cells. In IHC-P, the densest staining is seen in the perikarya of mouse brain tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRRK2

LRRK2 (also known as PARK8) is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. LRRK2 is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in LRRK2 have been associated with Parkinson disease.

Long Name

Leucine-rich Repeat Ser/Thr Protein Kinase 2

Alternate Names

AURA17, Dardarin, PARK8, ROCO2

Entrez Gene IDs

120892 (Human); 66725 (Mouse)

Gene Symbol

LRRK2

UniProt

Q5S007 (Human), Q5S006 (Mouse)

Additional LRRK2 Products

Product Documents for LRRK2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LRRK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.