LSD1 Antibody (1B2E5)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1762

Novus Biologicals, part of Bio-Techne
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NB100-1762
NB100-1762SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

Chemotaxis, ELISA, IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B2E5

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human LSD1 (between amino acids 400-600) expressed in E. coli. [UniProt# O60341]

Reactivity Notes

Human, mouse and monkey.

Localization

Nucleus

Marker

Nucleus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LSD1 Antibody (1B2E5)

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was evaluated immunohistochemically in a tissue microarray of 70 medulloblastomas (MB) and 9 tissue samples of normal cerebellum (CB). Micrograph showing KDM1A-positive staining in a representative MB sample, and KDM1A-negative staining in CB, scale bar=100AI1/4m. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
View 5 more images

Applications for LSD1 Antibody (1B2E5)

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Simple Western

1:500

Western Blot

1:500-1:2000
Application Notes
This LSD1 (1B2E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1

The amine oxidase domain 2 AOF2 gene encodes a nuclear protein (LSD1,~95 kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serves as a docking module for the stabilization of the associated corepressor complex(es) on chromatin.

Alternate Names

AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A

Entrez Gene IDs

23028 (Human)

Gene Symbol

KDM1A

UniProt

O60341 (Human)

Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products

Product Documents for LSD1 Antibody (1B2E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LSD1 Antibody (1B2E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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