LSD1 Antibody (1G7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15193
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1G7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for LSD1 Antibody (1G7)
Immunogen
A synthesized peptide derived from human LSD1. UniProt ID (O60341).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LSD1 Antibody (1G7)
Western Blot: LSD1 Antibody (1G7) [NBP3-15193]
Western Blot: LSD1 Antibody (1G7) [NBP3-15193] - Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate. All lanes: LSD1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 93, 96 KDa. Observed band size: 110 KDa.Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193]
Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193] - IHC image of LSD1 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193]
Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193] - IHC image of LSD1 antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for LSD1 Antibody (1G7)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1
Alternate Names
AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A
Gene Symbol
KDM1A
Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products
- All Products for Lysine (K)-specific Demethylase 1A/KDM1A/LSD1
- Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Activity Assays
- Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Lysates
- Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Primary Antibodies
- Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Proteins and Enzymes
Product Documents for LSD1 Antibody (1G7)
Product Specific Notices for LSD1 Antibody (1G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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