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LSD1 Antibody (1G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15193

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15193

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1G7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for LSD1 Antibody (1G7)

Immunogen

A synthesized peptide derived from human LSD1. UniProt ID (O60341).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LSD1 Antibody (1G7)

Western Blot: LSD1 Antibody (1G7) [NBP3-15193]

Western Blot: LSD1 Antibody (1G7) [NBP3-15193]

Western Blot: LSD1 Antibody (1G7) [NBP3-15193] - Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate. All lanes: LSD1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 93, 96 KDa. Observed band size: 110 KDa.
Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193]

Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193]

Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193] - IHC image of LSD1 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193]

Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193]

Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193] - IHC image of LSD1 antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for LSD1 Antibody (1G7)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1

LSD1 a recently identified, first known lysine-specific histone demethylase, is an 886 amino acid nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports suggest that it plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2 it serves as a novel biomarker predictive for prostate cancer.

Alternate Names

AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A

Gene Symbol

KDM1A

Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products

Product Documents for LSD1 Antibody (1G7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LSD1 Antibody (1G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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