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LSD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18670

Novus Biologicals, part of Bio-Techne
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NBP3-18670

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

2 mg/ml

Product Summary for LSD1 Antibody

Immunogen

Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using the full length recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for LSD1 Antibody

Western Blot: LSD1 Antibody [NBP3-18670]

Western Blot: LSD1 Antibody [NBP3-18670]

Western Blot: LSD1 Antibody [NBP3-18670] - Figure 1. Western blot analysis using the antibody directed against LSD1 Western blot was performed on nuclear extracts from HeLa cells (20 ug) using the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Western Blot: LSD1 Antibody [NBP3-18670]

Western Blot: LSD1 Antibody [NBP3-18670]

Western Blot: LSD1 Antibody [NBP3-18670] - Figure 2. Western blot analysis using the antibody directed against LSD1 Whole cell extracts (40 ug) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Applications for LSD1 Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

2 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1

LSD1 a recently identified, first known lysine-specific histone demethylase, is an 886 amino acid nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports suggest that it plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2 it serves as a novel biomarker predictive for prostate cancer.

Alternate Names

AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A

Gene Symbol

KDM1A

UniProt

Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products

Product Documents for LSD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LSD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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