LST1 Antibody (LST1/02) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45072

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LST1/02

Format

BSA Free

Concentration

1 mg/ml

View all LST1 Antibodies »

Product Summary for LST1 Antibody (LST1/02) - BSA Free

Immunogen

KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1

Specificity

The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LST1 Antibody (LST1/02) - BSA Free

Western Blot: LST1 Antibody (LST1/02)BSA Free [NBP1-45072]

Western Blot: LST1 Antibody (LST1/02)BSA Free [NBP1-45072]

Western Blot: LST1 Antibody (LST1/02) [NBP1-45072] - Analysis (non-reducing conditions) of LST1 in whole cell lysate of U937 human Caucasian histiocytic lymphoma cell line. Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-06) Lane 2-3: immunostaining with anti-human LST1 (LST1/02), two independent batches. Note: It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

Applications for LST1 Antibody (LST1/02) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western Blotting - It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LST1

LST1 (leukocyte-specific transcript, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A R11; LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.

Alternate Names

B144D6S49ELST-1, leucocyte specific transcript 1, leukocyte specific transcript 1, leukocyte-specific transcript 1 protein, lymphocyte antigen 117, MGC119006, MGC119007, Protein B144

Gene Symbol

LST1

Additional LST1 Products

Product Documents for LST1 Antibody (LST1/02) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LST1 Antibody (LST1/02) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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