Lunatic Fringe Antibody (1F9)

Novus Biologicals, part of Bio-Techne | Catalog # H00003955-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lunatic Fringe/LFNG Antibodies »

Product Specifications

Immunogen

LFNG (AAH14851, 1 a.a. ~ 250 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTPGRCCLAADIQVETFIFTDGEDEALARHTGNVVITNCSAAHSRQALSCKMAVEYDRFIESGRKWFCHVDDDNYVNLRALLRLLASYPHTRDVYVGKPSLDRPIQAMERVSENKVRPVHFWFATGGAGFCISRGLALKMSPWASGGHFMNTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTSELHEQVTLSYGMFENKRNAVHVKGPFSVEADPSRFRSIHCHLYPDTPWCPRTAIF

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

LFNG (1F9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Lunatic Fringe Antibody (1F9)

Western Blot: Lunatic Fringe Antibody (1F9) [H00003955-M03]

Western Blot: Lunatic Fringe Antibody (1F9) [H00003955-M03]

Western Blot: Lunatic Fringe Antibody (1F9) [H00003955-M03] - Analysis of LFNG expression in NIH/3T3 (Cat # L018V1).
ELISA: Lunatic Fringe Antibody (1F9) [H00003955-M03]

ELISA: Lunatic Fringe Antibody (1F9) [H00003955-M03]

ELISA: Lunatic Fringe Antibody (1F9) [H00003955-M03] - Detection limit for recombinant GST tagged LFNG is approximately 3ng/ml as a capture antibody.

Applications for Lunatic Fringe Antibody (1F9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lunatic Fringe/LFNG

This gene encodes a member of the glycosyltransferase superfamily. The encoded protein is a single-pass type II Golgi membrane protein that functions as a fucose-specific glycosyltransferase, adding an N-acetylglucosamine to the fucose residue of a group of signaling receptors involved in regulating cell fate decisions during development. Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Alternatively spliced transcript variants that encode different isoforms have been described, however, not all variants have been fully characterized.

Long Name

LFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase

Alternate Names

EC 2.4.1.222, LFNG, SCDO3

Entrez Gene IDs

3955 (Human)

Gene Symbol

LFNG

OMIM

602576 (Human)

UniProt

AAH14851 (Human)

Additional Lunatic Fringe/LFNG Products

Product Documents for Lunatic Fringe Antibody (1F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lunatic Fringe Antibody (1F9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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