Ly-6A/E Antibody (4N5K2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15949

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15949-100ul
NBP3-15949-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4N5K2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ly-6A/E Antibodies »

Product Summary for Ly-6A/E Antibody (4N5K2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse Ly-6A/E (P05533). MDTSHTTKSCLLILLVALLCAERAQGLECYQCYGVPFETSCPSITCPYPDGVCVTQEAAVIVDSQTRKVKNNLCLPICPPNIESMEILGTKVNVKTSCCQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ly-6A/E Antibody (4N5K2)

Western Blot: Ly-6A/E Antibody (4N5K2) [NBP3-15949]

Western Blot: Ly-6A/E Antibody (4N5K2) [NBP3-15949]

Western Blot: Ly-6A/E Antibody (4N5K2) [NBP3-15949] - Western blot analysis of extracts of various cell lines, using Ly-6A/E antibody (NBP3-15949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for Ly-6A/E Antibody (4N5K2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ly-6A/E

Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. (1-3) It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. (4-6) In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. (1, 7-9) The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. (1, 8, 9) Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses, (9-12) and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. (3, 13)

Alternate Names

Ly 6A/E, Ly6A.2, Ly6A/E, Ly6E.1, Lymphocyte antigen 6 complex locus A, Lymphocyte antigen 6 complex locus E, Retinoic acid induced gene E, RIGE, SCA1, SCA-1, SCA2, Stem cell antigen 1, Stem cell antigen 2, T cell activating protein, TAP, Thymic shared antigen 1, TSA1

Gene Symbol

LY6A

Additional Ly-6A/E Products

Product Documents for Ly-6A/E Antibody (4N5K2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ly-6A/E Antibody (4N5K2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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