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Ly-6A/E Antibody (RM0330-2M39) [Alexa Fluor® 405]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11871AF405

Novus Biologicals, part of Bio-Techne
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NBP2-11871AF405

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Rat IgG2 Clone # RM0330-2M39

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse recombinant Ly-6A/E

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Applications for Ly-6A/E Antibody (RM0330-2M39) [Alexa Fluor® 405]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Ly-6A/E

Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. (1-3) It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. (4-6) In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. (1, 7-9) The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. (1, 8, 9) Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses, (9-12) and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. (3, 13)

Alternate Names

Ly 6A/E, Ly6A.2, Ly6A/E, Ly6E.1, Lymphocyte antigen 6 complex locus A, Lymphocyte antigen 6 complex locus E, Retinoic acid induced gene E, RIGE, SCA1, SCA-1, SCA2, Stem cell antigen 1, Stem cell antigen 2, T cell activating protein, TAP, Thymic shared antigen 1, TSA1

Gene Symbol

LY6A

Additional Ly-6A/E Products

Product Documents for Ly-6A/E Antibody (RM0330-2M39) [Alexa Fluor® 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ly-6A/E Antibody (RM0330-2M39) [Alexa Fluor® 405]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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