Ly-6E Antibody (HL1933) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25553
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1933
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Ly-6E Antibody (HL1933) - Azide and BSA Free
Immunogen
Recombinant fragment of human Ly-6E
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ly-6E Antibody (HL1933) - Azide and BSA Free
Immunohistochemistry-Paraffin: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] -
Immunohistochemistry-Paraffin: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - LY6E antibody [HL1933] detects LY6E protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. LY6E stained by LY6E antibody [HL1933] (NBP3-25553) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] -
Western Blot: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (NBP3-25553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Knockdown Validated: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] -
Knockdown Validated: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - Non-transfected (-) and transfected (+) A549 whole cell extract (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (NBP3-25553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Ly-6E Antibody (HL1933) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ly6E
Long Name
Lymphocyte Antigen 6 Family Member E
Alternate Names
Ly-6E, RIGE, SCA2, TSA1
Gene Symbol
LY6E
Additional Ly6E Products
Product Documents for Ly-6E Antibody (HL1933) - Azide and BSA Free
Product Specific Notices for Ly-6E Antibody (HL1933) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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