Ly-6E Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03570
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Ly-6E Antibody - BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-101 of human Ly-6E (NP_002337.1). LMCFSCLNQKSNLYCLKPTICSDQDNYCVTVSASAGIGNLVTFGHSLSKTCSPACPIPEGVNVGVASMGISCCQSFLCNFS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ly-6E Antibody - BSA Free
![Western Blot: Ly-6E AntibodyBSA Free [NBP3-03570]](https://resources.bio-techne.com/images/products/Ly-6E-Antibody-Western-Blot-NBP3-03570-img0002.jpg "Western Blot: Ly-6E AntibodyBSA Free [NBP3-03570]")
Western Blot: Ly-6E AntibodyBSA Free [NBP3-03570]
Western Blot: Ly-6E Antibody [NBP3-03570] - Analysis of extracts of various cell lines, using Ly-6E antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.![Immunocytochemistry/ Immunofluorescence: Ly-6E Antibody - BSA Free [NBP3-03570]](https://resources.bio-techne.com/images/products/Ly-6E-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03570-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Ly-6E Antibody - BSA Free [NBP3-03570]")
Immunocytochemistry/ Immunofluorescence: Ly-6E Antibody - BSA Free [NBP3-03570]
Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP3-03570] - Analysis of L929 cells using LY6E Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Ly-6E antibody![Immunohistochemistry-Paraffin: Ly-6E Antibody - BSA Free [NBP3-03570]](https://resources.bio-techne.com/images/products/Ly-6E-Antibody-Immunohistochemistry-Paraffin-NBP3-03570-img0003.jpg "Immunohistochemistry-Paraffin: Ly-6E Antibody - BSA Free [NBP3-03570]")
Immunohistochemistry-Paraffin: Ly-6E Antibody - BSA Free [NBP3-03570]
Immunohistochemistry-Paraffin: Ly-6E Antibody [NBP3-03570] - Left panel shows negative control antibody on mouse kidney; right panel shows Ly-6E antibody at 1:100 dilution on mouse kidney. Brown = DAB reaction. Mouse kidneys were deparaffinized through xylenes and alcohols, boiled in citrate buffer for 25 minutes, then PBS-Tween at room temp for 5 minutes, followed by Scytek CRF anti-polyvalent polymer DAB kit protocol. Slides were counterstained for 30 seconds in hematoxylin and coverslipped. Image from verified customer review.Applications for Ly-6E Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
Validated from a verified customer review
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP3-03570 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ly6E
Long Name
Lymphocyte Antigen 6 Family Member E
Alternate Names
Ly-6E, RIGE, SCA2, TSA1
Gene Symbol
LY6E
Additional Ly6E Products
Product Documents for Ly-6E Antibody - BSA Free
Product Specific Notices for Ly-6E Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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