Ly-6G/Ly-6C Antibody (NIMP-R14)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1387
![Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]](https://resources.bio-techne.com/images/products/Ly-6G6C-Antibody-NIMP-R14-Immunohistochemistry-NB600-1387-img0006.jpg "Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]")
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Functional, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # NIMP-R14
Concentration
0.1 mg/ml
Product Specifications
Immunogen
Purified BALB/c mouse neutrophils
Epitope
This antibody recognizes a currently undetermined structure on the Neutrophil membrane
Specificity
This monoclonal antibody is highly specific for murine Ly-6G and Ly-6C.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Ly-6G/Ly-6C Antibody (NIMP-R14)
Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]
Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Histopathology of lung sections collected at 24 h pbi from mice co-infected with mouse adapted influenza A/Puerto Rico/8/34 (H1N1) (PR8) and type 2 pneumococcal strain D39 variants. Serial lung sections were subjected to immunohistochemistry (IHC) for pneumococcus using Rabbit Anti-Streptococcus pneumoniae Polyclonal Antibody (Catalog # NB100-64502) at 4 ug/mL or neutrophils using Rat Anti-Ly-6G/Ly-6C Monoclonal Antibody (Catalog # NB600-1387) at 2 ug/mL. Representative images at 4x magnification with 60x magnification inset are shown. Images courtesy of Dr. Amanda P. Smith, UTHSC, TN //doi.org/10.1101/659557![Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]](https://resources.bio-techne.com/images/products/Ly-6G6C-Antibody-NIMP-R14-Immunohistochemistry-Frozen-NB600-1387-img0005.jpg "Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]")
Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]
Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Staining for Ly-6G/Ly-6C FITC of frozen section of mouse spleen.Applications for Ly-6G/Ly-6C Antibody (NIMP-R14)
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:50
Immunohistochemistry-Paraffin
1:50
Application Notes
The monoclonal antibody NIMP-R14 has been succesfully used to stain neutrophils for fluorescent activated cell sorting and in frozen and paraffin sections. ICC/IF reactivity reported in scientific literature (PMID: 32543747).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS containing 0.1% BSA
Preservative
No Preservative
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Ly-6G (Gr-1)
Long Name
A Myeloid Differentiation Antigen
Alternate Names
Gr-1
Gene Symbol
LY6G
Additional Ly-6G (Gr-1) Products
Product Documents for Ly-6G/Ly-6C Antibody (NIMP-R14)
Product Specific Notices for Ly-6G/Ly-6C Antibody (NIMP-R14)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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