Ly-6G/Ly-6C Antibody (RM0064-6D17) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11784
![Immunohistochemistry-Paraffin: Ly-6G/Ly-6C Antibody (RM0064-6D17) - Azide and BSA Free [NBP2-11784]](https://resources.bio-techne.com/images/products/Ly-6G-Antibody-RM0064-6D17-Immunohistochemistry-Paraffin-NBP2-11784-img0001.jpg "Immunohistochemistry-Paraffin: Ly-6G/Ly-6C Antibody (RM0064-6D17) - Azide and BSA Free [NBP2-11784]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2 Clone # RM0064-6D17
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Cell lysates of mouse neutrophils Ig
Specificity
This antibody detects mouse Ly-6G (Gr-1), the LY-6G/LY-6C complex.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2
Scientific Data Images for Ly-6G/Ly-6C Antibody (RM0064-6D17) - Azide and BSA Free
Immunohistochemistry-Paraffin: Ly-6G/Ly-6C Antibody (RM0064-6D17) - Azide and BSA Free [NBP2-11784]
Immunohistochemistry-Paraffin: Ly-6G Antibody (RM0064-6D17) [NBP2-11784] - Staining of paraffin sections of mouse kidney and spleen tissues from LPS exposed animals.Applications for Ly-6G/Ly-6C Antibody (RM0064-6D17) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:500-1:2000
Immunohistochemistry
1:100-400
Immunohistochemistry-Paraffin
1:100-1:400
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Reconstitution
Reconstitute with sterilized PBS to a final concentration of 0.4 mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ly-6G (Gr-1)
Expression of Ly-6G and Ly-6C, along with other lineage markers, help define specific cell types (2,4). For example, mouse myeloid derived suppressor cells (MDSCs), which play a role in inflammation and tumor development, are further subdivided into granulocytic (G-MDSCs) or monocytic (Mo-MDSCs) (4). Mo-MDSCs express Ly-6Chi along with CD11b, but are negative for Ly-6G, whereas G-MDSCs express Ly-6Clo/int,Ly-6Ghi , and CD11b (4).
The monoclonal antibody RB6-8C5 first identified granulocyte-differentiation antigen (Gr-1) which is primarily expressed on the surface of neutrophils and immature myeloid cells, and to a lesser extent on lymphocytes and macrophages (1). Gr-1 expression increases as neutrophils mature but remains is stably expressed in monocytes (1). The RB6-8C5 reacts with both Ly-6G and Ly-6C, whereas 1A8 antibody is specific for Ly-6G (1). Antibodies against Ly-6G, such as RB6-8C5 and 1A8, are commonly used to identify a role for neutrophils in mouse models of disease as they are efficient for quickly depleting neutrophils (1). RB6-8C5, however, also targets and depletes Ly-6Chi monocytes in addition to other Ly-6G-expressing cells (1).
References
1. Lee, P. Y., Wang, J. X., Parisini, E., Dascher, C. C., & Nigrovic, P. A. (2013). Ly6 family proteins in neutrophil biology. Journal of leukocyte biology. https://doi.org/10.1189/jlb.0113014
2. Bamezai A. (2004). Mouse Ly-6 proteins and their extended family: markers of cell differentiation and regulators of cell signaling. Archivum immunologiae et therapiae experimentalis.
3. Loughner, C. L., Bruford, E. A., McAndrews, M. S., Delp, E. E., Swamynathan, S., & Swamynathan, S. K. (2016). Organization, evolution and functions of the human and mouse Ly6/uPAR family genes. Human genomics. https://doi.org/10.1186/s40246-016-0074-2
4. Kong, Y. Y., Fuchsberger, M., Xiang, S. D., Apostolopoulos, V., & Plebanski, M. (2013). Myeloid derived suppressor cells and their role in diseases. Current medicinal chemistry. https://doi.org/10.2174/0929867311320110006
Long Name
A Myeloid Differentiation Antigen
Alternate Names
Gr-1
Gene Symbol
LY6G
Additional Ly-6G (Gr-1) Products
Product Documents for Ly-6G/Ly-6C Antibody (RM0064-6D17) - Azide and BSA Free
Product Specific Notices for Ly-6G/Ly-6C Antibody (RM0064-6D17) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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