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Ly-6H Antibody (3E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00004062-M01

Novus Biologicals, part of Bio-Techne
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H00004062-M01

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

LY6H (AAH28894, 26 a.a. ~ 140 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LWCQDCTLTTNSSHCTPKQCQPSDTVCASVRITDPSSSRKDHSVNKMCASSCDFVKRHFFSDYLMGFINSGILKVDVDCCEKDLCNGAAGAGHSPWALAGGLLLSLGPALLWAGP

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23860910 and 25716842).

Localization

Cell membrane. Lipid-anchor, GPI-anchor.

Specificity

LY6H - lymphocyte antigen 6 complex, locus H

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ly-6H Antibody (3E10)

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - Short-term nicotine exposure does not alter Lypd6, Lynx1, or Ly6H levels in the brain. Lypd6, Lynx1, Ly6H, and Beta2 nAChR subunit protein levels were analyzed in (a) frontal cortex (FC) and hippocampal (HIP) tissue from rats administered nicotine (0.4 mg/kg s.c., twice daily) or vehicle (0.9% saline) for 7 days from day 8-14 or (b) 54-60 (n = 8). (c and d) Representative images of western blots summarized in (a) and (b), respectively. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jnc.13718) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Ly-6H Antibody (3E10) [H00004062-M01]

Immunohistochemistry-Paraffin: Ly-6H Antibody (3E10) [H00004062-M01]

Immunohistochemistry-Paraffin: Ly-6H Antibody (3E10) [H00004062-M01] - Analysis of monoclonal antibody to LY6H on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 2 ug/ml.
Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - Ly-6H in mouse Ly-6H proteins using anti-Ly-6H antibody. Image from verified customer review.

Applications for Ly-6H Antibody (3E10)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been used for IHC-P and ELISA. Use in ICC reported in scientific literature (PMID: 25716842). Use in immunoprecipitation reported in scientific literature (PMID: 25716842). Use in Immunohistochemistry reported in scientific literature (PMID: 25716842).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using H00004062-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ly-6H

LY6H (Lymphocyte antigen 6 complex, Locus H) is a member of the LY6 antigens family of glycosylphosphatidylinositol-anchored cell surface glycoproteins.

Alternate Names

Ly-6H, lymphocyte antigen 6 complex, locus H, lymphocyte antigen 6H, NMLY6

Entrez Gene IDs

4062 (Human)

Gene Symbol

LY6H

OMIM

603625 (Human)

Additional Ly-6H Products

Product Documents for Ly-6H Antibody (3E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ly-6H Antibody (3E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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