Lyn Antibody (LYN-01) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-519
![Western Blot: Lyn Antibody (LYN-01)BSA Free [NB500-519]](https://resources.bio-techne.com/images/products/Lyn-Antibody-LYN-01-Western-Blot-NB500-519-img0001.jpg "Western Blot: Lyn Antibody (LYN-01)BSA Free [NB500-519]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # LYN-01
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Bacterially expressed recombinant fragment of human Lyn, aa 8 - 238 (NM_001111097.2).
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
The LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Lyn Antibody (LYN-01) - BSA Free
Western Blot: Lyn Antibody (LYN-01)BSA Free [NB500-519]
Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane 3: JURKAT human peripheral blood T cell leukemia cell line Lane 4: A-431 human epidermoid carcinoma cell line Lane 5: U-937 human histiocytic lymphoma cell line.![Lyn Antibody (LYN-01) - BSA Free Western Blot: Lyn Antibody (LYN-01) - BSA Free [NB500-519]](https://resources.bio-techne.com/images/products/nb500-519_mouse-monoclonal-lyn-antibody-lyn-01-13120241643337.jpg "Western Blot: Lyn Antibody (LYN-01) - BSA Free [NB500-519]")
Western Blot: Lyn Antibody (LYN-01) - BSA Free [NB500-519]
Analysis of human LYN using mouse monoclonal antibody LYN-01 on lysates of Raji cell line and Jurkat cell line (LYN non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LYN monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LYN was detected around 55 kDa.Applications for Lyn Antibody (LYN-01) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:50
Western Blot
1-2 ug/ml
Application Notes
For WB: Use non-reducing conditions. Purity is > 95% (by SDS-PAGE).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NB500-519 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Lyn
Long Name
V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog
Alternate Names
Hck-2, JTK8, v-yes-1
Entrez Gene IDs
4067 (Human)
Gene Symbol
LYN
UniProt
Additional Lyn Products
Product Documents for Lyn Antibody (LYN-01) - BSA Free
Product Specific Notices for Lyn Antibody (LYN-01) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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