LYPLA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92089

Novus Biologicals, part of Bio-Techne
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NBP1-92089
NBP1-92089-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LYPLA3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGWLMRQDTEGLVEATMPPGVQLHCLYGTGVPTPDSFYYESFPDRDPKICFGDGDGTVNLKSALQCQAWQSRQEHQVLL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LYPLA3 Antibody

Immunohistochemistry-Paraffin: LYPLA3 Antibody [NBP1-92089]

Immunohistochemistry-Paraffin: LYPLA3 Antibody [NBP1-92089]

Immunohistochemistry-Paraffin: LYPLA3 Antibody [NBP1-92089] - Staining of human colon, kidney, lymph node and placenta using Anti-PLA2G15 antibody NBP1-92089 (A) shows similar protein distribution across tissues to independent antibody NBP1-92088 (B).
Western Blot: LYPLA3 Antibody [NBP1-92089]

Western Blot: LYPLA3 Antibody [NBP1-92089]

Western Blot: LYPLA3 Antibody [NBP1-92089] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: LYPLA3 Antibody [NBP1-92089]

Immunohistochemistry-Paraffin: LYPLA3 Antibody [NBP1-92089]

Immunohistochemistry-Paraffin: LYPLA3 Antibody [NBP1-92089] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
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Applications for LYPLA3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LYPLA3

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities.

Alternate Names

1-O-acylceramide synthase, ACSDKFZp564A0122, EC 2.3.1.43, GXVPLA2, LLPLLCAT-like lysophospholipase, LPLA2group XV phospholipase A2, LYPLA3EC 2.3.1.-, Lysophospholipase 3, lysophospholipase 3 (lysosomal phospholipase A2), Lysosomal phospholipase A2, phospholipase A2, group XV

Gene Symbol

PLA2G15

Additional LYPLA3 Products

Product Documents for LYPLA3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LYPLA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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