LYPLAL1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92090
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PMKGGISNVWFDRFKITNDCPEHLESIDVMCQVLTDLIDEEVKSGIKKNRILIGGFSMGGCMAMHLAYRNHQDVAGVFALSSF
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LYPLAL1 Antibody
Immunocytochemistry/ Immunofluorescence: LYPLAL1 Antibody [NBP1-92090]
Immunocytochemistry/Immunofluorescence: LYPLAL1 Antibody [NBP1-92090] - Staining of human cell line U-251 MG shows positivity in cytoplasm.Immunohistochemistry-Paraffin: LYPLAL1 Antibody [NBP1-92090]
Immunohistochemistry-Paraffin: LYPLAL1 Antibody [NBP1-92090] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules and in glomeruli.Simple Western: LYPLAL1 Antibody [NBP1-92090]
Simple Western: LYPLAL1 Antibody [NBP1-92090] - Lane view shows a specific band for LYPLA1 using 200 ug/mL of Sk-Mel-28 cell lysate and 1:4 antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 32 kDa. Image from an internal validation.Applications for LYPLAL1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Simple Western
1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. See Simple Western Antibody Database for Simple Western validation: tested in SK-MEL-28; antibody dilution of 1:20; separated by size; detects a band at 32 kDa
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LYPLAL1
Long Name
Lysophospholipase-like protein 1
Alternate Names
EC 3.1.2.-, FLJ99730, KIAA1238, lysophospholipase-like 1, lysophospholipase-like protein 1, Q96AV0
Gene Symbol
LYPLAL1
Additional LYPLAL1 Products
Product Documents for LYPLAL1 Antibody
Product Specific Notices for LYPLAL1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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