I have been looking for anti PLOD2 antibody to perform IHC on human lung cancer tissue. I have tried some antibodies from different companies, but it did not work for us well. I found this antibody cat# H00005352-B01P at Novus Biologicals website. On the data sheet you reported it for ICC and IF. I have been using your different antibodies in the past and had some satisfying results. May I ask you, please, if you had tried IHC on human tissue and it did not work or you have not tried it at all?

Name: Lysine Hydroxylase 2/PLOD2 Antibody
Brand: Novus Biologicals
SKU: H00005352-B01P
Rating: 5 (1 reviews)

H00005352-B01P is a product we distribute for a Taiwanese company called Abnova. We therefore do not do any in-house testing or development of their products. Abnova has validated this PLOD2 antibody for use in western via detection of the recombinant protein and overexpression transfected lysates. They have also validated this antibody in ICC with HeLa cells. They have not yet tested this antibody in tissue samples. See their datasheet here for all information we have regarding this product. Since this is not our product, we unfortunately cannot offer free samples. If you did want to try this product in an IHC experiment, we could offer you our Innovator's Reward Program. In exchange for a review of the product in an untested application or species, we would issue you a credit for the purchase price of the antibody for use in a future purchase. See details here Innovators Reward Program.

Lysine Hydroxylase 2/PLOD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00005352-B01P

(1)

Citations (3)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lysine Hydroxylase 2/PLOD2 Antibodies »

Product Specifications

Immunogen

PLOD2 (NP_891988.1, 1 a.a. - 758 a.a.) full-length human protein. MGGCTVKPQLLLLALVLHPWNPCLGADSEKPSSIPTDKLLVITVATKESDGFHRFMQSAKYFNYTVKVLGQGEEWRGGDGINSIGGGQKVRLMKEVMEHYADQDDLVVMFTECFDVIFAGGPEEVLKKFQKANHKVVFAADGILWPDKRLADKYPVVHIGKRYLNSGGFIGYAPYVNRIVQQWNLQDNDDDQLFYTKVYIDPLKREAINITLDHKCKIFQTLNGAVDEVVLKFENGKARAKNTFYETLPVAINGNGPTKILLNYFGNYVPNSWTQDNGCTLCEFDTVDLSAVDVHPNVSIGVFIEQPTPFLPRFLDILLTLDYPKEALKLFIHNKEVYHEKDIKVFFDKAKHEIKTIKIVGPEENLSQAEARNMGMDFCRQDEKCDYYFSVDADVVLTNPRTLKILIEQNRKIIAPLVTRHGKLWSNFWGALSPDGYYARSEDYVDIVQGNRVGVWNVPYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTANYNTSHYNNDLWQIFENPVDWKEKYINRDYSKIFTENIVEQPCPDVFWFPIFSEKACDELVEEMEHYGKWSGGKHHDSRISGGYENVPTDDIHMKQVDLENVWLHFIREFIAPVTLKVFAGYYTKGFALLNFVVKYSPERQRSLRPHHDASTFTINIALNNVGEDFQGGGCKFLRYNCSIESPRKGWSFMHPGRLTHLHEGLPVKNGTRYIAVSFIDP

Reactivity Notes

Rat reactivity reported in customer review.

Specificity

PLOD2 - procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Lysine Hydroxylase 2/PLOD2 Antibody

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P]

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P] - Analysis of PLOD2 expression in human kidney.

Immunocytochemistry/ Immunofluorescence: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P]

Immunocytochemistry/Immunofluorescence: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P] - Analysis of purified antibody to PLOD2 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P]

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P] - Analysis of PLOD2 expression in transfected 293T cell line by PLOD2 polyclonal antibody. Lane 1: PLOD2 transfected lysate(83.38 KDa). Lane 2: Non-transfected lysate.

Applications for Lysine Hydroxylase 2/PLOD2 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Reviewed Applications

Read 1 review rated 5 using H00005352-B01P in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysine Hydroxylase 2/PLOD2

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

LH2, PLOD2, TLH

Entrez Gene IDs

5352 (Human)

Gene Symbol

PLOD2

UniProt

NP_891988.1 (Human)

Additional Lysine Hydroxylase 2/PLOD2 Products

Product Documents for Lysine Hydroxylase 2/PLOD2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Lysine Hydroxylase 2/PLOD2 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

Lysine Hydroxylase 2/PLOD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00005352-B01P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all Lysine Hydroxylase 2/PLOD2 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Specificity

Clonality

Host

Isotype

Description

Scientific Data Images for Lysine Hydroxylase 2/PLOD2 Antibody

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P]

Immunocytochemistry/ Immunofluorescence: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P]

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P]

Applications for Lysine Hydroxylase 2/PLOD2 Antibody

Immunocytochemistry/ Immunofluorescence

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Lysine Hydroxylase 2/PLOD2

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Lysine Hydroxylase 2/PLOD2 Products

Product Documents for Lysine Hydroxylase 2/PLOD2 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Lysine Hydroxylase 2/PLOD2 Antibody