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Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72553

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5B9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human KDM4C expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9)Azide and BSA Free [NBP2-72553]

Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9)Azide and BSA Free [NBP2-72553]

Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JMJD2C(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JMJD2C.
Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]

Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]

Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553] - Staining of paraffin-embedded Ovary tissue using anti-JMJD2C mouse monoclonal antibody.
Flow Cytometry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]

Flow Cytometry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]

Flow Cytometry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY JMJD2C.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 4C/KDM4C

JMJD2C is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma.

Alternate Names

GASC1, JHDM3C, JMJD2C, KDM4C, Lysine (K)specific Demethylase 4C

Gene Symbol

KDM4C

Additional Lysine (K)-specific Demethylase 4C/KDM4C Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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