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Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32495

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32495

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH03-97

Concentration

1 mg/ml

Product Summary for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

Immunogen

Synthetic peptide within Human Lysine (K)-specific Demethylase 5B/Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B aa 960-1,009 / 1,544. (Uniprot: Q9UGL1)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

176 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495]

Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495] - Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B was immunoprecipitated in 0.2mg A549 cell lysate with NBP3-32495 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32495 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: A549 cell lysate (input)
Lane 2: IP in A549 cell lysate
Lane 3: Rabbit IgG in A549 cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds
Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495] - Western blot analysis of Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B antibody (NBP3-32495) at 1/2,000 dilution.

Lane 1: BT-20 cell lysate
Lane 2: MDA-MB-468 cell lysate
Lane 3: AGS cell lysate
Lane 4: A549 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 176 kDa
Observed band size: 145 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32495) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.

Applications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

Application
Recommended Usage

Immunoprecipitation

1-2ug/sample

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Gene Symbol

KDM5B

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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