Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84352

Novus Biologicals, part of Bio-Techne
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NBP1-84352
NBP1-84352-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, Chemotaxis, IF/IHC, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lysine (K)-specific Demethylase 5B/KDM5B Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSE

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24802759).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Staining in human testis and skeletal muscle tissues . Corresponding KDM5B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human skin shows moderate to strong nuclear positivity in epidermal cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts and Leydig cells.
View 3 more images

Applications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Immunoprecipitation

Reported in scientific literature (PMID: 25190814)

Western Blot

Reported in scientific literature (PMID: 25190814)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Gene Symbol

KDM5B

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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