Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97310

Novus Biologicals, part of Bio-Techne
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NBP1-97310
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.828 mg/ml

View all Lysine (K)-specific Demethylase 5B/KDM5B Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human JARID1B protein (between residues 800-850) [UniProt Q9UGL1]

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-97310]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-97310]

Immunocytochemistry/Immunofluorescence: JARID1B Antibody [NBP1-97310] - Jarid1B / KDM5B antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-97310]

Immunohistochemistry: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-97310]

Immunohistochemistry: JARID1B Antibody [NBP1-97310] - IHC analysis of JARID1B in human breast cancer xenograft using DAB with hematoxylin counterstain.

Applications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

reported in scientific literature (PMID 23354547)
Application Notes
In ICC/IF, staining of the nucleus was seen in HeLa cells. In paraffin, nuclear staining was observed in human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.828 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

Jumonji AT-rich interactive domain 1B (JARID1B) also known as Jumonji/ARID domain-containing protein 1B, Lysine-specific demethylase 5B (KDM5B), Cancer/testis antigen 31 (CT31), Histone demethylase JARID1B, PLU-1, and Retinoblastoma-binding protein 2 homolog 1 (RBP2-H1), is a member of the JARID 1 family. The JARID 1 proteins (JARID1A, JARID1B, JARID1C and JARID1D) catalyze the demethylation of histones on lysine, and JARID1B specifically demethylates lysine 4 on Histone H3. JARID1B functions as a transcriptional co-repressor for FOXG1B and PAX9. In addition, JARID1B may play a considerable role in cancer research as it assists in the propagation of breast cancer cells by repressing the tumor suppressor genes BRCA1 and HOXA5. Conversely, JARID1B acts as a direct suppressor of melanoma. As result, the JARID1B protein is up regulated in breast cancer and down regulated in melanoma and glioblastomas. In general, JARID1B expression is ubiquitous; with the highest expression seen in testis. JARID1B is subject to post translational phosphorylation and has two known isoforms; isoform 1: 175.6kDa and isoform 2: 179.4kDa.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Entrez Gene IDs

10765 (Human)

Gene Symbol

KDM5B

UniProt

Q9UGL1

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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