Lysozyme Antibody (LYZ/3942)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13961
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # LYZ/3942
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human Lysozyme protein (around aa 18-147) (exact sequence is proprietary) (Uniprot: P61626)
Localization
Secreted
Specificity
Lysozyme is an enzyme, commonly referred to as the body s own antibiotic since it kills bacteria. Natural substrate of lysozyme is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Lysozyme is synthesized predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. This antibody labels myeloid cells, histiocytes, granulocytes, macrophages and monocytes. It is helpful in the identification of myeloid or monocytic nature of acute leukemia.
Marker
Histiocytoma & Monocytic Acute Leukemia Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14114)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Lysozyme Antibody (LYZ/3942)
Western Blot: Lysozyme Antibody (LYZ/3942) [NBP3-13961]
Western Blot: Lysozyme Antibody (LYZ/3942) [NBP3-13961] - Western blot analysis of human spleen tissue and HepG2 cell lysates using Lysozyme Antibody (LYZ/3942).Immunohistochemistry-Paraffin: Lysozyme Antibody (LYZ/3942) [NBP3-13961]
Immunohistochemistry-Paraffin: Lysozyme Antibody (LYZ/3942) [NBP3-13961] - Formalin-fixed, paraffin-embedded human tonsil stained with Lysozyme antibody (LYZ/3942).Immunohistochemistry-Paraffin: Lysozyme Antibody (LYZ/3942) [NBP3-13961]
Immunohistochemistry-Paraffin: Lysozyme Antibody (LYZ/3942) [NBP3-13961] - Formalin-fixed, paraffin-embedded human spleen stained with Lysozyme antibody (LYZ/3942). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Lysozyme Antibody (LYZ/3942)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Lysozyme
Alternate Names
EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis
Gene Symbol
LYZ
UniProt
Additional Lysozyme Products
Product Documents for Lysozyme Antibody (LYZ/3942)
Product Specific Notices for Lysozyme Antibody (LYZ/3942)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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