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Lysozyme Antibody (ST50-02)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67507

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67507

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Multiplex Immunoassay, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST50-02

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within Human Lysozyme aa 36-85 / 148. (SwissProt: P61626 Human; SwissProt: P08905 Mouse)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysozyme Antibody (ST50-02)

Western Blot: Lysozyme Antibody (ST50-02) [NBP2-67507]

Western Blot: Lysozyme Antibody (ST50-02) [NBP2-67507]

Western Blot: Lysozyme Antibody (ST50-02) [NBP2-67507] - Western blot analysis of Lysozyme on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-
Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody (ST50-02) [NBP2-67507]

Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody (ST50-02) [NBP2-67507]

Immunocytochemistry/Immunofluorescence: Lysozyme Antibody (ST50-02) [NBP2-67507] - Staining Lysozyme in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Lysozyme Antibody (ST50-02) [NBP2-67507]

Immunohistochemistry-Paraffin: Lysozyme Antibody (ST50-02) [NBP2-67507]

Immunohistochemistry-Paraffin: Lysozyme Antibody (ST50-02) [NBP2-67507] - Analysis of paraffin-embedded mouse spleen tissue using anti-Lysozyme antibody. Counter stained with hematoxylin.

Applications for Lysozyme Antibody (ST50-02)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:200-1:500

Multiplex Immunoassay

Multiplex IHC: 1:2000

Western Blot

1:1000-1:2000
Application Notes
IP: Use at an assay dependent concentration.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysozyme

Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears.

Alternate Names

EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis

Gene Symbol

LYZ

Additional Lysozyme Products

Product Documents for Lysozyme Antibody (ST50-02)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lysozyme Antibody (ST50-02)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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