Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26165
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2E5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5)
Immunogen
A synthesized peptide derived from Human Lysyl Oxidase Homolog 2/LOXL2 [UniProt Q9Y4K0]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5)
Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5) [NBP3-26165] -
Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5) [NBP3-26165] - Image of Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5) [NBP3-26165] -
Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5) [NBP3-26165] - Image of Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5) [NBP3-26165] -
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5) [NBP3-26165] - Positive Western Blot detected in: MCF-7 whole cell lysate, PC3 whole cell lysate, Mouse brain tissue, Rat brain tissue.All lanes: Lysyl Oxidase Homolog 2/LOXL2 Antibody at 1:2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 87 kDa
Observed band size: 53 kDa
Applications for Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5)
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Lysyl Oxidase Homolog 2/LOXL2
Alternate Names
LOL2, LOXL2
Gene Symbol
LOXL2
Additional Lysyl Oxidase Homolog 2/LOXL2 Products
Product Documents for Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5)
Product Specific Notices for Lysyl Oxidase Homolog 2/LOXL2 Antibody (2E5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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