Lysyl Oxidase Homolog 2/LOXL2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32954

Novus Biologicals, part of Bio-Techne
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NBP1-32954
NBP1-32954-0.025ml
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Key Product Details

Validated by

Knockout/Knockdown, Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Feline

Cited:

Human

Predicted:

Bovine (96%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

IF/IHC, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lysyl Oxidase Homolog 2/LOXL2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lysyl Oxidase Homolog 2/LOXL2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (83%). Feline reactivity reported from a verified customer review.

Localization

Secreted, extracellular space

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lysyl Oxidase Homolog 2/LOXL2 Antibody

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human ovarian cancer. LOXL2 stained by LOXL2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:5000.
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Applications for Lysyl Oxidase Homolog 2/LOXL2 Antibody

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using NBP1-32954 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysyl Oxidase Homolog 2/LOXL2

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Alternate Names

LOL2, LOXL2

Entrez Gene IDs

4017 (Human); 94352 (Mouse)

Gene Symbol

LOXL2

UniProt

Q9Y4K0 (Human)

Additional Lysyl Oxidase Homolog 2/LOXL2 Products

Product Documents for Lysyl Oxidase Homolog 2/LOXL2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lysyl Oxidase Homolog 2/LOXL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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