Lysyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1719

Novus Biologicals, part of Bio-Techne
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NB100-1719
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Lysyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 597) of human Lysyl-tRNA Synthetase using the numbering given in entry NP_005539.1 (GeneID 3735).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysyl tRNA synthetase Antibody

Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719]

Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719]

Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Lysyl tRNA synthetase antibody NB100-1719 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719]

Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719]

Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Lysyl tRNA synthetase used at a dilution of 1:100.
Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719]

Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719]

Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for Lysyl tRNA synthetase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 -1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue section

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lysyl tRNA synthetase

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis

Alternate Names

CMTRIB, EC 6.1.1, EC 6.1.1.6, KARS2, KIAA0070, KRS, lysine tRNA ligase, Lysine--tRNA ligase, LysRS, lysyl-tRNA synthetase

Entrez Gene IDs

3735 (Human)

Gene Symbol

KARS1

UniProt

NP_005539.1 (Human)

Additional Lysyl tRNA synthetase Products

Product Documents for Lysyl tRNA synthetase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lysyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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