Lysyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32063

Novus Biologicals, part of Bio-Techne
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NBP1-32063
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lysyl tRNA synthetase Antibodies »

Product Summary for Lysyl tRNA synthetase Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lysyl tRNA synthetase. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (84%), Xenopus laevis (87%), Chicken (85%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lysyl tRNA synthetase Antibody

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-32063]

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-32063]

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-32063] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP1-32063]

Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP1-32063]

Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP1-32063] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-32063]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-32063]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-32063] - Paraffin-embedded mouse colon. Lysyl-tRNA synthetase antibody dilution: 1:50
View 1 more images

Applications for Lysyl tRNA synthetase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7), 40% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysyl tRNA synthetase

lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Alternate Names

CMTRIB, EC 6.1.1, EC 6.1.1.6, KARS2, KIAA0070, KRS, lysine tRNA ligase, Lysine--tRNA ligase, LysRS, lysyl-tRNA synthetase

Gene Symbol

KARS1

Additional Lysyl tRNA synthetase Products

Product Documents for Lysyl tRNA synthetase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lysyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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