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M-Cadherin/Cadherin-15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88101

Novus Biologicals, part of Bio-Techne
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NBP1-88101
NBP1-88101-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TFSARDPDTEQLQRLSYSKDYDPEDWLQVDAATGRIQTQHVLSPASPFLKGGWYRAIVLAQDDASQPRTATGTLSIEILEVNDHAPVLAPPPPGSLCSEPHQGPGLLLGATDEDLPPHGAPFHFQLSPRLPELGRNWSLSQVNVSH

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for M-Cadherin/Cadherin-15 Antibody

Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101]

Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101]

Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Analysis in human skeletal muscle and skin tissues. Corresponding CDH15 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: M-Cadherin/Cadherin-15 Antibody [NBP1-88101]

Immunocytochemistry/ Immunofluorescence: M-Cadherin/Cadherin-15 Antibody [NBP1-88101]

Immunocytochemistry/Immunofluorescence: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Staining of human cell line RH-30 shows localization to cytosol & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101]

Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101]

Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Staining of human cerebellum shows weak cytoplasmic positivity in cells in granular layer.

Applications for M-Cadherin/Cadherin-15 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: M-Cadherin/Cadherin-15

Cadherins are a multigene family of Ca++-dependent cell adhesion molecules. They are transmembrane glycoproteins consisting of an extracellular domain, which mediates Ca++-dependent intercellular adhesion by homophilic interactions, a transmembrane region and a cytoplasmic domain. The extracellular domain is divided into a series of subdomains designated EC1-EC5. Homolgies between different members of the cadherin family are most prominent in the cytoplasmic domain and in EC1 and EC2 and much less so in EC5 of the extracellular domain and in the transmembrane region. The binding properties and specificities of the adhesive function are located in the N-terminal part of the molecules. Four members of the cadherin family have been identified and molecularly cloned from mammalian cells. These include the neuronal (N), epithelial (E), placental (P) and muscle (M) cadherins. M-cadherin is not found in fibroblasts but is expressed at low level in myoblasts and is upregulated following induction of myotube formation, suggesting a specific function in skeletal muscle cell differentiation.

Long Name

M-Cadherin [Myotubule]

Alternate Names

Cadherin-15, CDH14, CDH15, CDHM, MCAD, MCadherin

Gene Symbol

CDH15

Additional M-Cadherin/Cadherin-15 Products

Product Documents for M-Cadherin/Cadherin-15 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for M-Cadherin/Cadherin-15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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