M-CSFR/CD115 Antibody (AFS98) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43363
![Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]](https://resources.bio-techne.com/images/products/M-CSF-R-CD115-Antibody-AFS98-Flow-Cytometry-NBP1-43363-img0002.jpg "Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]")
![Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]](https://resources.bio-techne.com/images/products/M-CSF-R-CD115-Antibody-AFS98-Flow-Cytometry-NBP1-43363-img0001.jpg "Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]")
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]](https://resources.bio-techne.com/images/products/M-CSFR-CD115-Antibody-AFS98-Immunohistochemistry-Paraffin-NBP1-43363-img0005.jpg "Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Block/Neutralize, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Block/Neutralize
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # AFS98
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was CD115.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for M-CSFR/CD115 Antibody (AFS98) - BSA Free
Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]
Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (AFS98) [NBP1-43363] - Mouse tumor tissue stained with M-CSFR/CD115 Antibody (AFS98). Tissue sections were deparaffinized and incubated with the antibody O/N at 4C. IHC-P image submitted by a verified customer review.![Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]](https://resources.bio-techne.com/images/products/M-CSF-R-CD115-Antibody-AFS98-Flow-Cytometry-NBP1-43363-img0003.jpg "Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]")
Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Analysis using the Biotin conjugate of NBP1-43363. Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram).![Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]](https://resources.bio-techne.com/images/products/M-CSFR-CD115-Antibody-AFS98-Immunohistochemistry-Frozen-NBP1-43363-img0004.jpg "Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]")
Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]
Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) [NBP1-43363] - Mouse brain section. Antibody at 1:200. IHC-Fr image submitted by a verified customer review.Applications for M-CSFR/CD115 Antibody (AFS98) - BSA Free
Application
Recommended Usage
Flow Cytometry
< 1 ug/10^6 cells
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Blocking/Neutralizing reported in scientific literature (PMID: 24056963). This M-CSFR/CD115 Antibody (AFS98) is validated for IHC-P from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP1-43363 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: M-CSF R/CD115
Long Name
Macrophage Colony Stimulating Factor Receptor
Alternate Names
c-fms, CD115, CSF1R, M-CSFR, MCSFR
Entrez Gene IDs
1436 (Human)
Gene Symbol
CSF1R
UniProt
Additional M-CSF R/CD115 Products
Product Documents for M-CSFR/CD115 Antibody (AFS98) - BSA Free
Product Specific Notices for M-CSFR/CD115 Antibody (AFS98) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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