M-CSFR/CD115 Antibody (1G4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00001436-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G4

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all M-CSF R/CD115 Antibodies »

Product Specifications

Immunogen

CSF1R (AAH47521, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFED

Specificity

CSF1R - colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for M-CSFR/CD115 Antibody (1G4) - Azide and BSA Free

Western Blot: M-CSFR/CD115 Antibody (1G4) [H00001436-M01]

Western Blot: M-CSFR/CD115 Antibody (1G4) [H00001436-M01]

Western Blot: M-CSFR/CD115 Antibody (1G4) [H00001436-M01] - CSF1R monoclonal antibody (M01), clone 1G4. Analysis of CSF1R expression in human colon.
Proximity Ligation Assay: M-CSFR/CD115 Antibody (1G4) [H00001436-M01]

Proximity Ligation Assay: M-CSFR/CD115 Antibody (1G4) [H00001436-M01]

Proximity Ligation Assay: M-CSFR/CD115 Antibody (1G4) [H00001436-M01] - Analysis of protein-protein interactions between GRAP2 and CSF1R. HeLa cells were stained with anti-GRAP2 rabbit purified polyclonal 1:1200 and anti-CSF1R mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: M-CSFR/CD115 Antibody (1G4) [H00001436-M01]

ELISA: M-CSFR/CD115 Antibody (1G4) [H00001436-M01]

ELISA: M-CSFR/CD115 Antibody (1G4) [H00001436-M01] - M-CSF R Antibody (1G4) [H00001436-M01] - Detection limit for recombinant GST tagged CSF1R is approximately 0.03ng/ml as a capture antibody.

Applications for M-CSFR/CD115 Antibody (1G4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: M-CSF R/CD115

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction.

Long Name

Macrophage Colony Stimulating Factor Receptor

Alternate Names

c-fms, CD115, CSF1R, M-CSFR, MCSFR

Entrez Gene IDs

1436 (Human)

Gene Symbol

CSF1R

OMIM

164770 (Human)

UniProt

AAH47521 (Human)

Additional M-CSF R/CD115 Products

Product Documents for M-CSFR/CD115 Antibody (1G4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for M-CSFR/CD115 Antibody (1G4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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