Macrophage Antibody (RM0029-11H3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12436

Novus Biologicals, part of Bio-Techne
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NBP2-12436-100ug
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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # RM0029-11H3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Macrophage Antibodies »

Product Specifications

Immunogen

Isolated mouse macrophages

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Macrophage Antibody (RM0029-11H3) - Azide and BSA Free

Immunohistochemistry-Paraffin: Macrophage Antibody (RM0029-11H3) - Azide and BSA Free [NBP2-12436]

Immunohistochemistry-Paraffin: Macrophage Antibody (RM0029-11H3) - Azide and BSA Free [NBP2-12436]

Immunohistochemistry-Paraffin: Macrophage Antibody (RM0029-11H3) [NBP2-12436] - Mouse skin tissue from human breast cancer cell xenograft.

Applications for Macrophage Antibody (RM0029-11H3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:100
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Macrophage

Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

Alternate Names

EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase

Additional Macrophage Products

Product Documents for Macrophage Antibody (RM0029-11H3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Macrophage Antibody (RM0029-11H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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