MAD2L1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-880

Novus Biologicals, part of Bio-Techne
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NB300-880
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MAD2L1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KVNSMVAYKIPVND corresponding to C-Terminus according to NP_002349.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MAD2L1 Antibody

Western Blot: MAD2L1 Antibody [NB300-880]

Western Blot: MAD2L1 Antibody [NB300-880]

Western Blot: Mad2L1 Antibody [NB300-880] - Analysis of Mad2L1 in HEK293 lysate (35ug protein in RIPA buffer) using NB300-880 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MAD2L1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

2 - 4 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in HEK293 lysates (calculated MW of 23.5 kDa band according to NP_002349.1). An additional band of unknown identity was also consistently observed at approx. 29 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAD2L1

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.

Long Name

MAD2 Mitotic Arrest Deficient-Like 1

Alternate Names

HSMAD2

Entrez Gene IDs

4085 (Human); 56150 (Mouse)

Gene Symbol

MAD2L1

UniProt

NP_002349. (Human)

Additional MAD2L1 Products

Product Documents for MAD2L1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAD2L1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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