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MAD2L2 Antibody (4D8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26158

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26158-100ul
NBP3-26158-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4D8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human MAD2L2 [UniProt Q9UI95]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAD2L2 Antibody (4D8)

MAD2L2 Antibody (4D8)

Immunohistochemistry: MAD2L2 Antibody (4D8) [NBP3-26158] -

Immunohistochemistry: MAD2L2 Antibody (4D8) [NBP3-26158] - Image of MAD2L2 Antibody (4D8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
MAD2L2 Antibody (4D8)

Western Blot: MAD2L2 Antibody (4D8) [NBP3-26158] -

Western Blot: MAD2L2 Antibody (4D8) [NBP3-26158] - Positive Western Blot detected in: 293 whole cell lysate.
All lanes: MAD2L2 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 25 kDa
Observed band size: 25 kDa
MAD2L2 Antibody (4D8)

Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (4D8) [NBP3-26158] -

Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (4D8) [NBP3-26158] - Staining of Hela Cells with MAD2L2 Antibody (4D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for MAD2L2 Antibody (4D8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MAD2L2

MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Alternate Names

hREV7, MAD2 mitotic arrest deficient-like 2 (yeast), MAD2BREV7 homolog, MAD2-like protein 2, Mitotic arrest deficient 2-like protein 2, mitotic arrest deficient homolog-like 2, mitotic spindle assembly checkpoint protein MAD2B, REV7yeast, homolog)-like 2

Gene Symbol

MAD2L2

Additional MAD2L2 Products

Product Documents for MAD2L2 Antibody (4D8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAD2L2 Antibody (4D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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