MAD2L2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-02798
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.68 mg/ml
Product Specifications
Immunogen
Synthetic peptide from human MAD2L2 corresponding to an N-terminus region.
Epitope
N-Terminus
Specificity
Aa 3-14 of Human MAD2L2. MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAD2L2 Antibody - BSA Free
![Western Blot: MAD2L2 AntibodyBSA Free [NBP1-02798]](https://resources.bio-techne.com/images/products/MAD2L2-Antibody-Western-Blot-NBP1-02798-img0003.jpg "Western Blot: MAD2L2 AntibodyBSA Free [NBP1-02798]")
Western Blot: MAD2L2 AntibodyBSA Free [NBP1-02798]
Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.![Immunohistochemistry-Paraffin: MAD2L2 Antibody - BSA Free [NBP1-02798]](https://resources.bio-techne.com/images/products/MAD2L2-Antibody-Immunohistochemistry-Paraffin-NBP1-02798-img0001.jpg "Immunohistochemistry-Paraffin: MAD2L2 Antibody - BSA Free [NBP1-02798]")
Immunohistochemistry-Paraffin: MAD2L2 Antibody - BSA Free [NBP1-02798]
Immunohistochemistry-Paraffin: MAD2L2 Antibody [NBP1-02798] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.Applications for MAD2L2 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:7500 - 1:30000
Immunocytochemistry/ Immunofluorescence
1:500 - 1:2000
Immunohistochemistry
10ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1:500 - 1:2000
Application Notes
.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
0.68 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MAD2L2
Alternate Names
hREV7, MAD2 mitotic arrest deficient-like 2 (yeast), MAD2BREV7 homolog, MAD2-like protein 2, Mitotic arrest deficient 2-like protein 2, mitotic arrest deficient homolog-like 2, mitotic spindle assembly checkpoint protein MAD2B, REV7yeast, homolog)-like 2
Entrez Gene IDs
10459 (Human)
Gene Symbol
MAD2L2
UniProt
Additional MAD2L2 Products
Product Documents for MAD2L2 Antibody - BSA Free
Product Specific Notices for MAD2L2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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