MADD Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77033

Novus Biologicals, part of Bio-Techne
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NBP1-77033
NBP1-77033SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all MADD Antibodies »

Product Summary for MADD Antibody - BSA Free

Immunogen

Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human MADD. The immunogen is located within the last 50 amino acids of MADD. Amino Acid Squence: AAVHSSEEDLRTPPRPVSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

200-220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MADD Antibody - BSA Free

Western Blot: MADD AntibodyBSA Free [NBP1-77033]

Western Blot: MADD AntibodyBSA Free [NBP1-77033]

Western Blot: MADD Antibody [NBP1-77033] - Whole cell lysates from the indicated cell lines with MADD antibody at 1:250 dilution.
Immunocytochemistry/ Immunofluorescence: MADD Antibody - BSA Free [NBP1-77033]

Immunocytochemistry/ Immunofluorescence: MADD Antibody - BSA Free [NBP1-77033]

Immunocytochemistry/Immunofluorescence: MADD Antibody [NBP1-77033] - Immunofluorescence of MADD in Hela cells with MADD antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: MADD Antibody - BSA Free [NBP1-77033]

Immunocytochemistry/ Immunofluorescence: MADD Antibody - BSA Free [NBP1-77033]

Immunocytochemistry/Immunofluorescence: MADD Antibody [NBP1-77033] - human spleen tissue with MADD antibody at 10 ug/ml.

Applications for MADD Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

1-2mg/mL
Application Notes
200 to 220 kDa bands should be detected in Western Blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MADD

MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94% overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87% identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.

Alternate Names

DENNFLJ36300, Differentially expressed in normal and neoplastic cells, FLJ35600, IG20, Insulinoma glucagonoma clone 20, insulinoma-glucagonoma protein 20, KIAA0358RAB3GEP, MAP kinase-activating death domain protein, MAP-kinase activating death domain, Rab3 GDP/GTP exchange factor

Gene Symbol

MADD

UniProt

AAD12154 (Human)

Additional MADD Products

Product Documents for MADD Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MADD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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